Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure
نویسندگان
چکیده
منابع مشابه
Advanced Methods in Fluorescence Microscopy
It requires a good deal of will power to resist hyperbole in considering the advances that have been achieved in fluorescence microscopy in the last 25 years. Our effort has been to survey the modalities of microscopic fluorescence imaging available to cell biologists and perhaps useful for diagnostic pathologists. The gamut extends from established confocal laser scanning through multiphoton a...
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The mitotic spindle is a cellular structure that guides proper chromosome segregation to daughter cells, thus ensuring genomic stability during cell proliferation. The mitotic spindle is made of microtubules, polymers of alpha and beta tubulin, and it is highly dynamic during the cell cycle, indeed it is build de novo and it is destroyed at each cell division. The spindle form, position and fun...
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Cell division requires replication of DNA followed by physical segregation of chromosomes and replicated chromatids. DNA replication is likely to leave duplicate sister chromatids wrapped around one another (catenated) at least once every few kilobases (Chapter 15). Then, as the cell enters mitosis, chromosomes are folded (“condensed”) into neatly compacted and segregated mitotic chromosomes ea...
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Nano-resolution fluorescence electron microscopy (nano-fEM) pinpoints the location of individual proteins in electron micrographs. Plastic sections are first imaged using a super-resolution fluorescence microscope and then imaged on an electron microscope. The two images are superimposed to correlate the position of labeled proteins relative to subcellular structures. Here, we describe the meth...
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Little study of insects with modern nanotechnology tools has been done so far. Here we use one of such tool, atomic force microscopy (AFM) to study surface oscillations of the ladybird beetles (Hippodamia convergens) measured in different parts of the insect at picometer level. This allows us to record a much broader spectral range of possible surface vibrations (up to several kHz) than the pre...
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ژورنال
عنوان ژورنال: Chromosome Research
سال: 2021
ISSN: 0967-3849,1573-6849
DOI: 10.1007/s10577-021-09654-5